Course title
アグリバイオ実験   [Basic experiments of biological production]
Course category   Requirement   Credit 2 
Department   Year 3  Semester Spring 
Course type Spring  Course code 01AN3113
Instructor(s)
山田 哲也   [YAMADA Tetsuya]
Facility affiliation Faculty of Agriculture Office   Email address

Course description
Based on biological experiment, chemical experiment, and basics of biological production experiment, further technical analysis and observation on the technical fields of production environment science, plant production science and animal / insect production science will be performed.
Expected Learning
Understand knowledge on advanced biological production, such as molecular biology, based on experiment.
Course schedule
1. Guidance
2. Extraction of plant pigments and observation of plant cells I
3. Extraction of plant pigments and observation of plant cells II
4. Genetic diagnosis in small samples
5. Quantification of a macronutrient and a low molecular compound in a plant I
6. Quantification of a macronutrient and a low molecular compound in a plant II
7. Quantification of a macronutrient and a low molecular compound in a plant III
8. Cultivation of soil microorganisms
9. Observation of soil microorganisms
10. Glycochemistry of mucopolysaccharide
11. Immunochemistry of mucin-related protein
12. Introduction of the gene into E. coli cells by heat shock method
13. PCR amplification of the transgene and introduction of the gene into plant cells using Agrobacterium
14. Electrophoresis of PCR amplification products and detection of transformed callus by GUS assay
15. Summary of the experiments
Prerequisites
Required Text(s) and Materials
References
Assessment/Grading
Message from instructor(s)
Course keywords
Office hours
Remarks 1
Remarks 2
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Last update
3/21/2017 7:57:58 PM