| Course title | |||||
| 環境資源科学実験Ⅲ(生物学応用) [Experiment on Environmental and Natural Resource Science III (Advanced Biology)] | |||||
| Course category | Requirement | Credit | 1 | ||
| Department | Year | 3～ | Semester | Spring | |
| Course type | Spring | Course code | 01EN3127 | ||
| Instructor(s) | |||||
| 多羅尾 光徳 [TARAO Mitsunori] | |||||
| Facility affiliation | Faculty of Agriculture | Office | Email address | ||
| Course description |
|
Participants of this course are expected to learn experimental techniques relating to biology necessary for environmental and natural resource science. Specific coverage will include as follows; (1) Effect of environmental stress on plants, and analysis of plant metabolic activities and inorganic constituents (2) Morphological characteristics of invertebrates (3) Mechanisms of formation of plant biomass and biological assessment and classification (4) Measurement of microorganisms in freshwater environments (5) Transformation of bacterium (6) Microbial degradation of xenobiotics (6) Biodegradation of plant biomass |
| Expected Learning |
|
1. To learn experimental techniques relating to biology necessary for environmental and natural resource sciences. 2. To learn scientific writings necessary for science. |
| Course schedule |
|
1. Effect of soil acidification caused by acid rain on plants (Dr. Izuta & Dr. M. Watanabe) Artificial acidification of two types of soil common in Japan, and investigation of changes in soil chemical properties and plant growth 2. Anatomical characteristics of invertebrates (Dr. Ohji & Dr. I. Watanabe) Dissection of fishes, and observation of structural characteristics and functional details of tissues and organs 3. Formation of Plants (biomass resource) and biological assessment, I: Mechanisms of tree growth (Dr. Funada & Dr. Nakaba) Observation of tree cells, such as, lateral meristem (cambium layer, cambial initial) for hypertrophic growth, xylem in the process of differentiation and matured cells, and cells organising outer bark and inner bark (phloem tissue), and understanding their anatomical characteristics and mechanisms of hypertrophic growth. 4. Formation of Plants (biomass resource) and biological assessment, II: Biologica assessment and classification of biomass resource (Dr. Funada & Dr. Nakaba) Participants understand that changes in density as the most critical physiological index for wood resource relates to structural ratio and form of cell types forming wood. 5. Distribution of microorganisms in freshwater environment (Dr. Katayama & Dr. Tarao) Measurement of viable cells, coliforms, and xenobiotics degraders in freshwater (rivers, streams, springs, and ponds) samples around Fuchu campus of TUAT. Microscopic observation of microorganisms in biofilm 6. Transformation of bacterium (Dr. Katayama & Dr. Tarao) Transformation of E. coli by a plasmid with genes for antibiotics resistance 7. Microbial degradation of xenobiotics (Dr. Katayama & Dr. Tarao) Measurement of the rate constant of degradation of p-nitrophenol by degrading microorganisms 8. Biodegradation of plants (biomass resource), I: Cultivation of fungi by using a medium with cellulose as a carbon source (Dr. Yoshida) Collecition of conidia of fungi are collected, and cultivation of fungi in cellulose medium 9. Biodegradation of plants (biomass resource), I: Measurement of activities of cellulose degrading enzyme in culture medium (Dr. Yoshida) Measurement of metabolic activity of cellulose degrading enzyme produced in culture medium |
| Prerequisites |
| Participants are assumed to have Biology, Microbiology, Fundamental Experiment on Biology, Microbial Physiology and Ecology, Environmental Microbiology, Environmental Botany, Plant Anatomy and Morphogenesis, Tree Eco-Physiology, Biodegradation (all are not compulsory). |
| Required Text(s) and Materials |
| Download an instruction handbook from the portal site (noodle). |
| References |
| "Safety manual", TUAT |
| Assessment/Grading |
| Score is evaluated based on reports. Attending all practice is required. |
| Message from instructor(s) |
|
Labo ware, indoor shoes, goggle, and writing implement are indispensable. Take care in case of dealing with chemicals. Obey instruction by teachers and teaching assistants. |
| Course keywords |
| plants and environmental stress; transformation of microorganism; plasmid, biodegrdation of biomass resource; formation, biological assessment and classification of biomass resource |
| Office hours |
| 10 - 12 a.m., Monday |
| Remarks 1 |
| Absence is not allowed except for illness, injury or a death in the family. |
| Remarks 2 |
| Related URL |
| http://www.tuat.ac.jp/~env-mic/studentpractice/biologyadvanced.html |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 3/7/2017 2:47:19 PM |