| Course title | |||||
| 微生物利用学特論 [Advanced Applied Microbiology] | |||||
| Course category | Requirement | Credit | 0.5 | ||
| Department | Year | ～ | Semester | Spring | |
| Course type | Spring | Course code | 96214 | ||
| Instructor(s) | |||||
| 久留主 泰朗（茨） [KURUSU Yasuroh] | |||||
| Facility affiliation | United Graduate School Agricultural Science | Office | Email address | ||
| Course description |
|
“Basic and Applied Biology of Plasmids” Plasmids serve as powerful tools in the expression of a specific enzyme and/or metabolite in the cultivation of microorganisms when combined with recombinant DNA technology. Especially, genetic manipulation of the genus Sphingomonas is necessary to improve the ability of these bacteria to degrade xenobiotic compounds and to elucidate the unique mechanisms involved in degradation. Strains of the genus Sphingomonas have a unique characteristic: they contain glycosphingolipids, which are ubiquitous in eukaryotic cell membranes. When Sphingomonas sp. strain A1 assimilates a macromolecule (alginate), a mouth-like pit is formed on the cell surface through reorganization and/or fluidity of the pleats, causing extracellular alginate to be concentrated in the pit. However, a plasmid suitable for genetic manipulation of Sphingomonas has not been identified. Some vector systems in sphingomonads with a broad-host-range plasmid or a cryptic plasmid have been reported. Also, transformation of sphingomonads by conjugation has been described. Here, we show the Plasmids Biology as follows. 1) Characterization of plasmid replication systems. 2) Characterization of plasmid partition systems and replication systems. 3)Construction of expression vector for various useful bacteria as hosts. |
| Expected Learning |
| Understanding of the basic theories of replication and partition mechanisms. Understanding how the plasmids are practically applied on basis of the lecture for various useful bacteria. |
| Course schedule |
|
1) Characterization of plasmid replication systems. 2) Characterization of plasmid partition systems and replication systems. 3) Construction of expression vector for various useful bacteria as hosts. |
| Prerequisites |
| Required Text(s) and Materials |
| Handouts will be provided in class. |
| References |
| To be announced in the lectures. |
| Assessment/Grading |
| Based on report. |
| Message from instructor(s) |
| I will devote a lecture to discussing a current, basic and applied science of plasmids. I believe that my talk is so basic that it will help your research activity in future. |
| Course keywords |
| Office hours |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Language Subject |
| Last update |
| 4/17/2017 3:52:45 PM |