Course title | |||||
アグリバイオ実験 [Basic experiments of biological production] | |||||
Course category | Requirement | Credit | 2 | ||
Department | Year | 3~ | Semester | Spring | |
Course type | Spring | Course code | 01AN3113 | ||
Instructor(s) | |||||
阿部 広明 [ABE Hiroaki] | |||||
Facility affiliation | Faculty of Agriculture | Office | Email address |
Course description |
Based on biological experiment, chemical experiment, and basics of biological production experiment, further technical analysis and observation on the technical fields of production environment science, plant production science and animal / insect production science will be performed. |
Expected Learning |
Understand knowledge on advanced biological production, such as molecular biology, based on experiment. |
Course schedule |
1. Guidance 2. Extraction of plant pigments and observation of plant cells I 3. Extraction of plant pigments and observation of plant cells II 4. Genetic diagnosis in small samples 5. Quantification of a macronutrient and a low molecular compound in a plant I 6. Quantification of a macronutrient and a low molecular compound in a plant II 7. Quantification of a macronutrient and a low molecular compound in a plant III 8. Cultivation of soil microorganisms 9. Observation of soil microorganisms 10. Glycochemistry of mucopolysaccharide 11. Immunochemistry of mucin-related protein 12. Introduction of the gene into E. coli cells by heat shock method 13. PCR amplification of the transgene and introduction of the gene into plant cells using Agrobacterium 14. Electrophoresis of PCR amplification products and detection of transformed callus by GUS assay 15. Summary of the experiments |
Prerequisites |
Required Text(s) and Materials |
References |
Assessment/Grading |
Message from instructor(s) |
Course keywords |
Office hours |
Remarks 1 |
Remarks 2 |
Related URL |
Lecture Language |
Language Subject |
Last update |
8/2/2018 11:03:54 PM |