Course title
細胞工学   [Cell Technology]
Course category		Requirement		Credit	2
Department		Year	3～	Semester	Fall
Course type	Fall	Course code	01BN3134
Instructor(s)
森山 裕充, 平岡 毅, 三浦 豊   [MORIYAMA Hiromitsu, HIRAOKA Tsuyoshi, MIURA Yutaka]
Facility affiliation	Faculty of Agriculture	Office		Email address

Course description

Learn about the characteristics of cell cultures of higher animals, insects and plants (including yeast), techniques, properties of cells, their application to biology, agriculture, etc.

Expected Learning

Learn the basic knowledge on cell engineering methods using animal, insect, plant (yeast) cells.

Course schedule

Mammalian cells Outline of cell culture Cell proliferation, animal cell culture, cell regulation factor Serum-free culture, cell fractionation and cloning Storage of cellular resources, hybridomas Intracellular injection, in vitro assay Learn the basics and applications mainly on mammalian cells. Insect cell History and development of insect cell culture Equipment necessary for insect cell culture, machine tools Method of primary culture of insect cells Continuous passaging cell lines derived from insects and their characteristics Application of insect cell culture Learn about the specialty of specialized insect cell culture, specific technology and field of use. Plant (yeast) cells Separation of callus culture protoplasts, culture and cell fusion Introduction of foreign genes into plant cells and breeding of transgenic plants and its application Substance production system using yeast cells Measurement and control of cell culture condition Measure cell concentration ・ Measure the number of viable cells Measurement and control of DO (Dissolved Oxygen Dissolved Oxygen) Handling of jar fermentor and control of cell proliferation

Prerequisites

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Office hours

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Last update

6/12/2018 8:10:18 PM