Course title
環境資源科学実験Ⅲ(生物学応用)   [Experiment on Environmental and Natural Resource Science III (Advanced Biology)]
Course category		Requirement		Credit	1
Department		Year	3～	Semester	Spring
Course type	Spring	Course code	01EN3127
Instructor(s)
多羅尾 光徳   [TARAO Mitsunori]
Facility affiliation	Faculty of Agriculture	Office		Email address

Course description

Participants of this course are expected to learn experimental techniques relating to biology necessary for environmental and natural resource science. Specific coverage will include as follows; 1. Environmental stress treatment on plants and growth analysis 2. Mechanism of biodegradation of plant biomass 3. Anatomical characteristics of vertebrates 4. Mechanisms of formation of plant biomass and biological assessment and classification 5. Transformation of bacterium 6. Microbial degradation of xenobiotics

Expected Learning

1. To learn experimental techniques relating to biology necessary for environmental and natural resource sciences. 2. To learn scientific writings necessary for science.

Course schedule

1. Environmental stress treatment on plants and growth analysis (Dr. Izuta & Dr. M. Watanabe) Plants are grown under the condition of water stress, and the growth and physiological responses of plants are analysed. 2. Mechanism of biodegradation of plant biomass (Dr. M. Yoshida) (1) Cultivation of fungi by using a medium with cellulose as a carbon source Fungal conidia are collected and cultivated in cellulose medium. (2) Measurement of activities of cellulose degrading enzyme in culture medium Metabolic activity of cellulose degrading enzyme produced in culture medium are measured. 3. Anatomical characteristics of vertebrates (Dr. Ohji & Dr. I. Watanabe) Dissection of fishes, and observation of structural characteristics and functional details of tissues and organs 4. Mechanisms of formation of plant biomass and biological assessment and classification (Dr. Funada & Dr. Nakaba) (1) Mechanisms of tree growth Observation of tree cells, such as, lateral meristem (cambium layer, cambial initial) for hypertrophic growth, xylem in the process of differentiation and matured cells, and cells organising outer bark and inner bark (phloem tissue), and understanding their anatomical characteristics and mechanisms of hypertrophic growth. (2) Biologica assessment and classification of biomass resource Participants understand that changes in density as the most critical physiological index for wood resource relates to structural ratio and form of cell types forming wood. 5. Transformation of bacterium (Dr. Tarao) Transformation of E. coli by a plasmid with genes for antibiotics resistance 6. Microbial degradation of xenobiotics (Dr. Tarao) Measurement of the rate constant of degradation of p-nitrophenol by degrading microorganisms

Prerequisites

Participants are assumed to have Biology, Microbiology, Fundamental Experiment on Biology, Microbial Physiology and Ecology, Environmental Microbiology, Environmental Botany, Plant Anatomy and Morphogenesis, Tree Eco-Physiology, Biodegradation (all are not compulsory).

Required Text(s) and Materials

Download an instruction handbook from the portal site (noodle).

References

"Safety manual", TUAT

Assessment/Grading

Score is evaluated based on reports. Attending all practice is required.

Message from instructor(s)

Labo ware, indoor shoes, goggle, and writing implement are indispensable. Take care in case of dealing with chemicals. Obey instruction by teachers and teaching assistants.

Course keywords

plants and environmental stress; transformation of microorganism; plasmid, biodegrdation of biomass resource; formation, biological assessment and classification of biomass resource

Office hours

10 - 12 a.m., Monday

Remarks 1

Absence is not allowed except for illness, injury or a death in the family.

Remarks 2

Related URL

http://www.tuat.ac.jp/~env-mic/studentpractice/biologyadvanced.html

Lecture Language

Japanese

Language Subject

Last update

3/30/2018 5:06:52 PM