Course title
植物遺伝育種学特論   [Advanced Plant Genetics and Breeding Science]
Course category   Requirement   Credit 2 
Department   Year 1  Semester Spring 
Course type Spring  Course code 05MP5137
Instructor(s)
山田 哲也   [YAMADA Tetsuya]
Facility affiliation Faculty of Agriculture Office 6-406  Email address

Course description
In this course, traditional breeding methods are initially overview. Then, principles and application examples of breeding techniques to assist those methods are explained in detail. In addition to marker-assisted selection and recombinant DNA techniques, new plant breeding techniques (NBT) such as genome editing using artificial nucleases and epigenome editing using RNA-depending DNA methylation are introduced.
Expected Learning
The goal of this course is that students will be able to understand the new breeding techniques used in plant breeding through learning of theory, principle, and application examples of them.
Course schedule
Unit 1: Orientation lecture
Unit 2: Traditional breeding methods (1), Summary of plant breeding, Mutation breeding
Unit 3: Traditional breeding methods (2), Hybridization breeding
Unit 4: Traditional breeding methods (3), Wide cross breeding
Unit 5: Traditional breeding methods (4), Polyploidy breeding
Unit 6: Traditional breeding methods (5), Marker-assisted selection
Unit 7: Traditional breeding methods (6), Recombinant DNA techniques, Cisgenesis/intragenesis
Unit 8: Genome editing (1), Summary of genome editing, Artificial nucleases
Unit 9: Genome editing (2), Meganuclease, Zinc finger nucleases (ZFNs)
Unit 10: Genome editing (3), Transcription activator-like effector nuclease (TALEN), Clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated gene (Cas) system
Unit 11: Genome editing (4), Oligonucleotide directed mutagenesis (ODM)
Unit 12: Epigenome editing (1), RNA-depending DNA methylation (RdDM)
Unit 13: Epigenome editing (2), Grafting on GM rootstock
Unit 14: Other techniques, Reverse breeding, Agro-infiltration
Prerequisites
It is desirable to take the courses of plant molecular genetics and plant breeding science in undergraduate school.
Required Text(s) and Materials
Reference materials will be distributed as needed.
References
Assessment/Grading
Degree of participation in class (50%) and small test of 10 times (50%)
Message from instructor(s)
Course keywords
Variety improvement, Breeding methods, Biotechnology, Genetic engineering, Artificial nuclease
Office hours
I will respond to questions and consultations at any time. It is also available in the E-mail.
Remarks 1
Remarks 2
Related URL
Lecture Language
Japanese
Language Subject
Last update
3/12/2018 6:57:27 PM