| Course title | |||||
| 分子生物学Ⅱ [Molecular Biology Ⅱ] | |||||
| Course category | Requirement | Credit | 2 | ||
| Department | Year | 2～ | Semester | 3rd | |
| Course type | 3rd | Course code | 01BN2117 | ||
| Instructor(s) | |||||
| 森山 裕充, 佐々木 信光, 松下 保彦 [MORIYAMA Hiromitsu, SASAKI Nobumitsu, MATSUSHITA Yasuhiko] | |||||
| Facility affiliation | Faculty of Agriculture | Office | Email address | ||
| Course description |
| Genetic research in the post-genome era aims at a comprehensive understanding of molecular and genetic levels of various life activities indicated by cells and individuals. Here, lecture on fundamental methods and concepts of molecular biology playing a central role in genetic research, and important research results. In the first half, we will give a lecture on molecular biology centered on yeast cells, which is a model of eukaryotes from a molecular genetic point of view, and lecture on research methods based on molecular biology and its application in the latter half. In the second half, we will give lectures on fundamental technologies concerning the handling of nucleic acids among research methods based on molecular biology. |
| Expected Learning |
|
We will make it possible to grasp from basic contents to current topics about DNA replication machinery and genetic manipulation. To be able to understand and explain the basic method of gene cloning. To be able to design oligonucleotides for DNA processing using PCR method. |
| Course schedule |
|
First half (Moriyama) 1. Transcription control by RNA polymerase II ？？Transcription regulation mechanism in yeast ？？Breeding of useful yeast by transcription machine engineering 2. RNA transport / metabolic control ？？Molecular breeding of mRNA flux in nucleus and cytoplasm ？？MRNA flux under ethanol stress 3. Signal transmission and cell proliferation regulation ？？Molecular control of signaling mechanism ？？Application of yeast as a model organism of pharmacology research 4. Glycobiology and glycosylation engineering Biosynthesis of sugar chains Glycan engineering of yeast 5. Function of mitochondria Function of mitochondria and its role in yeast Metabolic alteration based on mitochondrial function 6. Autophagy-related factors in yeast 7. Physiological roles of autophagy in yeast 8. test Latter half (Matsushita, Sasaki) 9. Cloning and cloning (vector, transformation, selection) 10. DNA processing technology (confirmation of basic knowledge, principle of PCR method) 11. Gene cloning technology (isolation of plasmid DNA, restriction enzyme, ligated enzyme) 12. Gene cloning technology (sequencing method, TA cloning, RACE method, inverse PCR method) 13. DNA pocessing using PCR method (deletion, fusion) 14. DNA processing using PCR method (insertion, mutation) 15. Plasmid processing using PCR method (utilization of inverse PCR the concept) |
| Prerequisites |
| Required Text(s) and Materials |
| Principles of genetic engineering (Sankyo Publishing, ISBN 978-4-7827-0637-4) |
| References |
| Assessment/Grading |
| Message from instructor(s) |
| Course keywords |
| Cloning, gene cloning, PCR method, DNA processing |
| Office hours |
| Please have an appointment after lecture or by e-mail. |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| http://www.tuat.ac.jp/~mcb/, http://web.tuat.ac.jp/~idenshi/ |
| Lecture Language |
| Japanese |
| Language Subject |
| English |
| Last update |
| 2/25/2019 12:40:05 PM |