| Course title | |||||
| 細胞工学 [Cell Technology] | |||||
| Course category | Requirement | Credit | 2 | ||
| Department | Year | 3～ | Semester | 3rd | |
| Course type | 3rd | Course code | 01BN3134 | ||
| Instructor(s) | |||||
| 森山 裕充, 平岡 毅, 三浦 豊 [MORIYAMA Hiromitsu, HIRAOKA Tsuyoshi, MIURA Yutaka] | |||||
| Facility affiliation | Faculty of Agriculture | Office | Email address | ||
| Course description |
| Learn about the characteristics of cell cultures of higher animals, insects and plants (including yeast), techniques, properties of cells, their application to biology, agriculture, etc. |
| Expected Learning |
|
Learn the basic knowledge on cell engineering methods using animal, insect, plant (yeast) cells. Corresponding criteria in the Diploma policy: See the curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/ |
| Course schedule |
|
Mammalian cells Outline of cell culture Cell proliferation, animal cell culture, cell regulation factor Serum-free culture, cell fractionation and cloning Storage of cellular resources, hybridomas Intracellular injection, in vitro assay Learn the basics and applications mainly on mammalian cells. Insect cell History and development of insect cell culture Equipment necessary for insect cell culture, machine tools Method of primary culture of insect cells Continuous passaging cell lines derived from insects and their characteristics Application of insect cell culture Learn about the specialty of specialized insect cell culture, specific technology and field of use. Plant (yeast) cells Separation of callus culture protoplasts, culture and cell fusion Introduction of foreign genes into plant cells and breeding of transgenic plants and its application Substance production system using yeast cells Measurement and control of cell culture condition Measure cell concentration ・ Measure the number of viable cells Measurement and control of DO (Dissolved Oxygen Dissolved Oxygen) Handling of jar fermentor and control of cell proliferation |
| Prerequisites |
|
It is desirable that you take the following subjects. Molecular biology I, Insect Biology, Molecular Cell Biology II, Plant Physiology |
| Required Text(s) and Materials |
| Occasionally prepare the latest research papers in the special field |
| References |
| Occasionally prepare the books in the special field |
| Assessment/Grading |
| Attitude to study on subnects (10%), presentation and final examination (90%) |
| Message from instructor(s) |
| Course keywords |
| Office hours |
| Weekdays 9: 00 ~ 18: 00, questions and discussions will be accepted as required. Emails are also acceptable. |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 3/5/2019 12:17:05 PM |