| Course title | |||||
| 分子生物学特別研究Ⅱ [Advanced Experiment on Molecular BiologyⅡ] | |||||
| Course category | thesis research and other related studies | Requirement | Credit | 4 | |
| Department | Year | 2～ | Semester | YearLong | |
| Course type | YearLong | Course code | 05ML5408 | ||
| Instructor(s) | |||||
| 山形 洋平 [YAMAGATA Yohei] | |||||
| Facility affiliation | Faculty of Agriculture | Office | afjgxte/L1151 | Email address | |
| Course description |
| Set own research theme in the Master's first year, and perform educational research along that theme. |
| Expected Learning |
| To master at least two types of research methods. |
| Course schedule |
|
Molecular biology and genetic engineering techniques Bacteria, yeast and fugi culture, nucleic acid extraction method, PCR ・ RT-PCR, sequencing, expression vector construction, agarose gel electrophoresis, real-time PCR Biochemical technology High-performance liquid chromatography, affinity chromatography, electrophoresis, Immuno blot / Northern blot method, various assay, enzyme activity measurement method, centrifugal separation method, density gradient than Centrifugation, between BiaCore molecular interaction analysis, chemical emission and fluorescence image detection method Cell biology techniques Mouse / human cell culture, transgenic expression, cell staining ・ time lapse fluorescence microscopy, induced expression / KO ・ establishment of stable cell lines, Xenopus breeding, microinjection, Tissue staining, RNAi / shRNA gene silencing Genomics technology MALDI-TOF mass spectrometry, peptide mass finger method, two-dimensional electrophoresis, image analysis software handling method, protein complex isolation methods, ESI-Q-TOF mass spectrometry, nano-LCMS / MS method, large-scale shotgun method, BIA-MS method, Mascot identification software analysis, stable isotope label quantitative analysis, large-scale identification information processing method, from among the microRNA analysis, Izu Re or to learn at least two types of research methods. |
| Prerequisites |
|
Related to subjects of the thesis, it is a required subject. The required learning time is based on the standard number of hours of our university. |
| Required Text(s) and Materials |
| Prepare based on the necessity |
| References |
| Prepare based on the necessity |
| Assessment/Grading |
| Evaluating submitted master thesis. |
| Message from instructor(s) |
| Course keywords |
| Research project, objective, method, analysis, discussion |
| Office hours |
| It is possible at any time. However, I recommend that you make an appointment by e-mail in advance. |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 3/6/2019 4:53:41 PM |