Course title
生体分子化学Ⅰ   [Biomolecular and Biomaterial Chemistry Ⅰ]
Course category		Requirement		Credit	2
Department		Year	1～2	Semester	1st
Course type	1st	Course code	05cc0001
Instructor(s)
北野 克和, 千葉 一裕, 松下 保彦, 佐々木 信光   [KITANO Yoshikazu, CHIBA Kazuhiro, MATSUSHITA Yasuhiko, SASAKI Nobumitsu]
Facility affiliation	Faculty of Agriculture	Office	Bio-organic Chemistry, Gene Research Center	Email address

Course description

This course provides students with the skills of determining the structure of low molecular-weight organic compounds. This course also provides students with the basics on analysis methods of gene expression and function including those of subcellular localization and dynamics of cellular macromolecules. We also describe various analytical methods on gene expression and function along with the basic idea underlying them. In addition, we explain the techniques, examples, and recent information about researches of subcellular localization and dynamics of cellular macromolecules.

Expected Learning

Learners who succesfully complete this course will be able to identify the structure of low molecular-weight organic compounds by analayzing IR, MS, and NMR spectra. Learners who succesfully complete this course will be able to understand the principle of various analytical methods concerning gene expression and function including analysis method of subcellular localization and dynamics of cellular macromolecules, along with the underlying basic concept. Corresponding criteria in the Diploma policy: See the curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/

Course schedule

Omnibus/15 periods total CHIBA Kazuhiro (3 periods) 1. Outline of determining the structure of an organic compound 2. Infrared spectroscopy (IR) and Mass spectrometry (MS) 3. Nuclear magnetic resonance spectroscopy (NMR) KITANO Yoshikazu (4 periods) 4. Strategy for determining the structure of an organic compound 5. Practice for determining the structure of an organic compound (1) 6. Practice for determining the structure of an organic compound (2) 7. Practice for determining the structure of an organic compound (3) MATSUSHITA Yasuhiko (4 periods) 8. Analysis method of gene expression and function 9. Analysis method of gene expression (1) RNA 10. Analysis method of gene expression (2) Protein 11. Analysis method of gene function SASAKI Nobumitsu (4 periods) 12. Basics of microscopic analysis of subcellular localization and dynamics of cellular macromolecules 13. Research examples of subcellular localization and dynamics of RNA molecules 14. Research examples of subcellular localization and dynamics of proteins 15. Recent research topics about subcellular localization and dynamics of cellular macromolecules

Prerequisites

This lecture is an elective subject, and is one of the specialized subjects. Students need to preview and review for an amount of time as indicated in the student guidelines of TUAT, in addition to the attendance of lecture (30 hours).

Required Text(s) and Materials

Handouts (paper and/or electronic) will be provided if needed.

References

Spectrometric Identification of Organic Compounds: Robert M. Silverstein, Francis X. Webster, David J. Kiemle, David L. Bryce (ISBN: 978-0-470-61637-6) Sankyo Publishing: Principle of Genetic Engineering (in Japanese) (ISBN: 978-4-7827-0637-4) Shujunsha: Fluorescence Imaging Revolution (ISBN: 978-4-7809-0835-0)

Assessment/Grading

Performance in lecture (Kitano; 10% total), examination (Chiba & Kitano; 40% total), report assignments (Matsushita & Sasaki; 50% total)

Message from instructor(s)

We hope that this lecture will help you on your research in the future.

Course keywords

Infrared spectroscopy, Mass spectrometry, Nuclear magnetic resonance spectroscopy, Next generation sequencer, RNA-seq, Yeast hybrid method, Fluorescent microscope, Confocal laser microscope, Bimolecular fluorescence complementation method

Office hours

Questions can be asked after class, or please request an appointment by email.

Remarks 1

Remarks 2

Related URL

Lecture Language

Japanese

Language Subject

Last update

2/21/2019 9:48:30 AM