| Course title | |||||
| 分析科学 [Analytical Science] | |||||
| Course category | Requirement | Credit | 2 | ||
| Department | Year | 3～ | Semester | 1st | |
| Course type | 1st | Course code | 01AN3105 | ||
| Instructor(s) | |||||
| 田中 治夫 [TANAKA Haruo] | |||||
| Facility affiliation | Faculty of Agriculture | Office | 2-221 (Tanaka) | Email address | |
| Course description |
|
Students will learn the analytical technique used in biological production. This lecture is the common subject of the Department of Biological Production. |
| Expected Learning |
|
Students who successfully complete this course will be able to explain and apply the methods of analytical chemistry, analysis of the population and the group, biochemical analysis of the protein, and molecular biological analysis of the gene. Corresponding criteria in the Diploma Policy: See the Curriculum maps. (URL: https://www.tuat.ac.jp/campuslife_career/campuslife/policy/) |
| Course schedule |
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Unit 1 (Tanaka, H.): Analytical chemistry (1), Spectrum analysis Unit 2 (Ohtsu): Analytical chemistry (2), Columns and chromatogram Unit 3 (Suzuki, S.): Molecular biological analysis of the gene (1), Southern Northern analysis, promoter analysis Unit 4 (Yamada, T.): Molecular biological analysis of the gene (2), Real-time PCR, RNAi method Unit 5 (Sato, K.): Molecular biological analysis of the gene (3), Cloning, DGGE Unit 6 (Okazaki): Molecular biological analysis of the gene (4), Metagenome and meta-transcriptome analysis Unit 7 (Tabunoki): Biochemical analysis of proteins (1), Functional analysis of the protein using affinity chromatography and SDS-PAGE Unit 8 (Sugimura): Biochemical analysis of proteins (2), Tissues and cells Imaging (Immunohistochemical staining, Immunocytochemical staining, Live cell imaging) Unit 9 (Ookawa): Analysis of the population and the group (1), QTL analysis Unit 10 (Yokoyama): Analysis of the population and the group (2), Genetic analysis of quantitative traits Unit 11 (Ito): Analysis of the population and the group (3), Positional cloning Unit 12 (Shimmura): Analysis of the population and the group (4), Behavioural analysis Unit 13 (Ban): Analysis of the population and the group (5) Image analysis of plant root systems Unit 14 (Motobayashi): Analysis of the population and the group (6), Population density Unit 15 (Katsura): Analysis of the population and the group (7), Carbon dynamics in crop canopies |
| Prerequisites |
| In addition to 30 hours that students spend in the class, students are recommended to prepare for and revise the lectures, spending the standard amount of time as specified by the University and using the lecture handouts as well as the references specified below. |
| Required Text(s) and Materials |
| Prints |
| References |
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Japanese texts 1: ハーウッド・クラリッジ1999：有機化合物のスペクトル解析入門，化学同人 2: 本浄高治ら1998：基礎分析化学，化学同人 3: 西方敬人・真壁和裕2005：超実践 バイオ実験イラストレイテッド レッスン1，キットも活用遺伝子実験，秀潤社 4: 中山広樹1999：バイオ実験イラストレイテッド③ 本当にふえるPCR，秀潤社 5: 真壁和裕1997：バイオ実験イラストレイテッド④ 苦労なしのクローニング，秀潤社，大藤道衛2004：電気泳動なるほどQ＆A，羊土社 6: 服部正平 2010：メタゲノム解析技術の最前線, シーエムシー出版 7: 長谷俊治・高尾敏文・高木淳一 2010：タンパク質科学実験法①，化学同人 8: 野地澄晴2006：免疫染色＆ in situ ハイブリダイゼーション最新プロトコール，羊土社 9: 鵜飼保雄2002：量的形質の遺伝解析，医学書院 10: 田島 弥太郎1993：蚕の品種育成 (昆虫利用科学シリーズ)，サイエンスハウス 11: 神村学ら2009:分子昆虫学-ポストゲノムの昆虫研究-, 共立出 12: 佐藤衆介ら2011：動物行動図説，朝倉書店 13: 木村和彦1998：画像解析による根長と根の直径の測定-その1.実践編-，根の研究，7：8-11 14: 伊藤嘉昭ら1992：動物生態学，蒼樹書房 15: 平沢正・大杉立2016：農学基礎シリーズ 作物生産生理学の基礎，農文協 |
| Assessment/Grading |
| Attitude to the class (40%) and semester test (60%) |
| Message from instructor(s) |
| Course keywords |
| Analytical chemistry, molecular biological analysis of genes, biochemical analysis of proteins, analysis of populations |
| Office hours |
| By e-mail |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 2/20/2020 5:35:11 PM |