Course title
応用生物科学専門実験Ⅴ   [Laboratory Work of Applied Biological Science Ⅴ]
Course category   Requirement   Credit 1 
Department   Year 3  Semester 1st 
Course type 1st  Course code 01BN3158
Instructor(s)
小松 健, 松下 保彦   [KOMATSU Ken, MATSUSHITA Yasuhiko]
Facility affiliation Faculty of Agriculture Office afjgxte/L1151  Email address

Course description
A class: Nutritional physiological chemistry and experiment in cell engineering.
Students are expected to master handling of biological samples, and aseptic procedure (sterile culture) via the experiments relevant to nutritional physiological chemistry and cell engineering. Measurement of the net weight and purity of certain protein within the food intake, isolation of rat skin-derived fibroblast cells, growth analysis of culture cells and immune blotting with specific antibody will be planned/provided.
B class: Experiment on insect physiology, plant disease and tissue culture will be provided.

Expected Learning
A class: Nutritional physiological chemistry and experiment in cell engineering.
Students are expected to master handling of biological samples, and sterilized culture method via the experiments relevant to nutritional physiological chemistry and cell engineering. Measurement of the net weight and purity of certain protein within the food intake, isolation of rat skin-derived fibroblast cells, growth analysis of culture cells and experiment using specific antibody will be planned/provided.
B class: Experiment on insect physiology, plant disease and tissue culture will be provided.
Transfer of culture cells: learn aseptic procedure (sterile culture).
Isolate primary cells from animal body and observe the cells.
Observation of chromosome of culture cells and discuss on the increase of chromosome during the culture period.
Experiment on plant disease/pathogen: isolate, culture and observe plant pathogen.

Corresponding criteria in the Diploma policy:
See the curriculum maps.
https://www.tuat.ac.jp/campuslife_career/campuslife/policy/
Course schedule
A class: Nutritional physiological chemistry and experiment in cell engineering.
Students are expected to master handling of biological samples, and sterilized culture method via the experiments relevant to nutritional physiological chemistry and cell engineering. Measurement of the net weight and purity of certain protein within the food intake, isolation of rat skin-derived fibroblast cells, growth analysis of culture cells and experiment using specific antibody will be planned/provided.
B class: Experiment on insect physiology, plant disease and tissue culture will be provided.
Transfer of culture cells: learn aseptic procedure (sterile culture).
Isolate primary cells from animal body and observe the cells.
Observation of chromosome of culture cells and discuss on the increase of chromosome during the culture period.
Experiment on plant disease/pathogen: isolate, culture and observe plant pathogen.
Prerequisites
In addition to the lectures in the class, you are recommended to prepare and review the class using the standard amount of time as specified by the university for each class.
Required Text(s) and Materials
Please refer to the syllabus in Japanese.
References
Please refer to the syllabus in Japanese.
Assessment/Grading
Grade will be decided by the report submitted during the course (100%).
Message from instructor(s)
Course keywords
Office hours
Please ask faculties in charge of the experiments.
Remarks 1
Remarks 2
Related URL
Lecture Language
Japanese
Language Subject
Last update
2/29/2020 12:42:53 PM