| Course title | |||||
| 環境資源科学実験Ⅲ(生物学応用) [Experiment on Environmental and Natural Resource Science III (Advanced Biology)] | |||||
| Course category | Requirement | Credit | 1 | ||
| Department | Year | 3～ | Semester | 1st | |
| Course type | 1st | Course code | 01EN3127 | ||
| Instructor(s) | |||||
| 多羅尾 光徳 [TARAO Mitsunori] | |||||
| Facility affiliation | Faculty of Agriculture | Office | afjgxte/L1151 | Email address | |
| Course description |
|
Participants of this course are expected to learn experimental techniques relating to biology necessary for environmental and natural resource science. Specific coverage will include as follows; 1. Environmental stress treatment on plants and growth analysis 2. Mechanism of biodegradation of plant biomass 3. Anatomical characteristics of vertebrates 4. Mechanisms of formation of plant biomass and biological assessment and classification 5. Transformation of bacterium 6. Microbial degradation of xenobiotics |
| Expected Learning |
|
1. To learn experimental techniques relating to biology necessary for environmental and natural resource sciences. 2. To learn scientific writings necessary for science. Corresponding criteria in the Diploma Policy. See the Curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/ |
| Course schedule |
|
1. Environmental stress treatment on plants and growth analysis (Dr. Izuta & Dr. M. Watanabe) Plants are grown under the condition of water stress, and the growth and physiological responses of plants are analysed. 2. Mechanism of biodegradation of plant biomass (Dr. M. Yoshida) (1) Cultivation of fungi by using a medium with cellulose as a carbon source Fungal conidia are collected and cultivated in cellulose medium. (2) Measurement of activities of cellulose degrading enzyme in culture medium Metabolic activity of cellulose degrading enzyme produced in culture medium are measured. 3. Anatomical characteristics of vertebrates (Dr. Ohji & Dr. I. Watanabe) Dissection of fishes, and observation of structural characteristics and functional details of tissues and organs 4. Mechanisms of formation of plant biomass and biological assessment and classification (Dr. Funada & Dr. Nakaba) (1) Mechanisms of tree growth Observation of tree cells, such as, lateral meristem (cambium layer, cambial initial) for hypertrophic growth, xylem in the process of differentiation and matured cells, and cells organising outer bark and inner bark (phloem tissue), and understanding their anatomical characteristics and mechanisms of hypertrophic growth. (2) Biologica assessment and classification of biomass resource Participants understand that changes in density as the most critical physiological index for wood resource relates to structural ratio and form of cell types forming wood. 5. Transformation of bacterium (Dr. Tarao) Transformation of E. coli by a plasmid with genes for antibiotics resistance 6. Microbial degradation of xenobiotics (Dr. Tarao) Measurement of the rate constant of degradation of p-nitrophenol by degrading microorganisms |
| Prerequisites |
|
- Participants are assumed to have Biology, Microbiology, Fundamental Experiment on Biology, Microbial Physiology and Ecology, Environmental Microbiology, Environmental Botany, Plant Anatomy and Morphogenesis, Tree Eco-Physiology, Biodegradation (all are not compulsory). - In completing this course, students will spend the standard amount of time as specified by the University. |
| Required Text(s) and Materials |
| Download an instruction handbook from the portal site (noodle). |
| References |
| "Safety manual", TUAT |
| Assessment/Grading |
| Score is evaluated based on reports. Attending all practice is required. |
| Message from instructor(s) |
|
Labo ware, indoor shoes, goggle, and writing implement are indispensable. Take care in case of dealing with chemicals. Obey instruction by teachers and teaching assistants. |
| Course keywords |
| plants and environmental stress; transformation of microorganism; plasmid, biodegrdation of biomass resource; formation, biological assessment and classification of biomass resource |
| Office hours |
| Tarao: 10 - 12 a.m., weekdays |
| Remarks 1 |
| Absence is not allowed except for illness, injury or a death in the family. |
| Remarks 2 |
| Related URL |
| http://www.tuat.ac.jp/~env-mic/studentpractice/biologyadvanced.html |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 3/31/2020 4:47:46 PM |