Course title
生命工学実験Ⅱ   [Experiment for Biotechnology and Life Science Ⅱ]
Course category technology speciality courses  Requirement   Credit 4 
Department   Year 24  Semester 3rd 
Course type 3rd  Course code 022177
Instructor(s)
平田 美智子   [HIRATA Michiko]
Facility affiliation Faculty of Engineering Office afjgxte/L1151  Email address

Course description
This experiment aim is to master basic experimental techniques based on essential study for engineering science. We will study wide range of biotechnology techniques and form the right solution of research data. Topics include organic chemistry practice, physical chemistry practice, molecular biology practice, biochemistry practice, and instrumental analysis.
Expected Learning
In molecular biology - biochemistry practice, we will study basic method of microorganisms and cloning technique. In organic chemistry - physical chemistry practice, we will study various organic chemistry reactions and how to make the reactions. In addition, we will learn data processing in instrumental analysis.
Course schedule
1. Introduction and risk management
2. Molecular biology - biochemistry practice
A. Culture and morphological observation of microorganisms
I. Measuring the number of living microorganisms on nutrient agar
II. Morphological observation of microorganisms with a microscope
B. Cloning for introducing the desired foreign gene
I. Separation and purification of GFP gene
II. Cloning for GFP gene
III. Check the result of cloning for GFP gene with PCR
and culture of transformant
IV. Purification of cloned gene
3. Organic chemistry - physical chemistry practice
I. Introduction and risk management in this practice
II. Reduction reaction of aldehyde to alcohol
III. Ene reaction of carbonyl compound
IV. Purification with column chromatography / Reduction reaction of Olefin
V. Separation and purification of menthol
VI. Synthesis of dipeptide using mixed acid anhydrides
VII. Separation and purification of dipeptide
VIII. Check the compound with Infrared Spectrophotometry
4. Instrumental analysis
I. Information processing for life science related data using internet
II. Programming and run of Python programming language
III. Mathematical calculation analysis
Prerequisites
You are required to have related subject’s knowledge.
Required Text(s) and Materials
Experiment for Biotechnology and life Science II textbook
References
We will show you appropriate reference books as needed.
Assessment/Grading
Total evaluation with Experiment participation, Experiment notes, Reports
Message from instructor(s)
You will work collaboratively throughout the course to solve problems and work on your reports. You will find a delight in making experiments in this course.
Course keywords
Organic chemistry, physical chemistry, molecular biology, biochemistry, and instrumental analysis.
Office hours
Remarks 1
Remarks 2
Related URL
Lecture Language
Language Subject
Last update
12/17/2019 1:27:04 PM