| Course title | |||||
| 生命工学実験Ⅱ [Experiment for Biotechnology and Life Science Ⅱ] | |||||
| Course category | technology speciality courses | Requirement | Credit | 4 | |
| Department | Year | 2～4 | Semester | 3rd | |
| Course type | 3rd | Course code | 022177 | ||
| Instructor(s) | |||||
| 平田 美智子 [HIRATA Michiko] | |||||
| Facility affiliation | Faculty of Engineering | Office | afjgxte/L1151 | Email address | |
| Course description |
| This experiment aim is to master basic experimental techniques based on essential study for engineering science. We will study wide range of biotechnology techniques and form the right solution of research data. Topics include organic chemistry practice, physical chemistry practice, molecular biology practice, biochemistry practice, and instrumental analysis. |
| Expected Learning |
| In molecular biology - biochemistry practice, we will study basic method of microorganisms and cloning technique. In organic chemistry - physical chemistry practice, we will study various organic chemistry reactions and how to make the reactions. In addition, we will learn data processing in instrumental analysis. |
| Course schedule |
|
1. Introduction and risk management 2. Molecular biology - biochemistry practice A. Culture and morphological observation of microorganisms I. Measuring the number of living microorganisms on nutrient agar II. Morphological observation of microorganisms with a microscope B. Cloning for introducing the desired foreign gene I. Separation and purification of GFP gene II. Cloning for GFP gene III. Check the result of cloning for GFP gene with PCR and culture of transformant IV. Purification of cloned gene 3. Organic chemistry - physical chemistry practice I. Introduction and risk management in this practice II. Reduction reaction of aldehyde to alcohol III. Ene reaction of carbonyl compound IV. Purification with column chromatography / Reduction reaction of Olefin V. Separation and purification of menthol VI. Synthesis of dipeptide using mixed acid anhydrides VII. Separation and purification of dipeptide VIII. Check the compound with Infrared Spectrophotometry 4. Instrumental analysis I. Information processing for life science related data using internet II. Programming and run of Python programming language III. Mathematical calculation analysis |
| Prerequisites |
| You are required to have related subject’s knowledge. |
| Required Text(s) and Materials |
| Experiment for Biotechnology and life Science II textbook |
| References |
| We will show you appropriate reference books as needed. |
| Assessment/Grading |
| Total evaluation with Experiment participation, Experiment notes, Reports |
| Message from instructor(s) |
| You will work collaboratively throughout the course to solve problems and work on your reports. You will find a delight in making experiments in this course. |
| Course keywords |
| Organic chemistry, physical chemistry, molecular biology, biochemistry, and instrumental analysis. |
| Office hours |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Language Subject |
| Last update |
| 12/17/2019 1:27:04 PM |