| Course title | |||||
| 生体分子化学Ⅰ [Biomolecular and Biomaterial Chemistry Ⅰ] | |||||
| Course category | Requirement | Credit | 2 | ||
| Department | Year | 1～ | Semester | 1st | |
| Course type | 1st | Course code | 05lc0101 | ||
| Instructor(s) | |||||
| 北野 克和, 千葉 一裕, 松下 保彦, 佐々木 信光 [KITANO Yoshikazu, CHIBA Kazuhiro, MATSUSHITA Yasuhiko, SASAKI Nobumitsu] | |||||
| Facility affiliation | Faculty of Agriculture | Office | afjgxte/L1151 | Email address | |
| Course description |
|
This course provides students with the skills of determining the structure of low molecular-weight organic compounds. This course also provides students with the basics on analysis methods of gene expression and function including those of subcellular localization and dynamics of cellular macromolecules. We also describe various analytical methods on gene expression and function along with the basic idea underlying them. In addition, we explain the techniques, examples, and recent information about researches of subcellular localization and dynamics of cellular macromolecules. |
| Expected Learning |
|
Learners who succesfully complete this course will be able to identify the structure of low molecular-weight organic compounds by analayzing IR, MS, and NMR spectra. Learners who succesfully complete this course will be able to understand the principle of various analytical methods concerning gene expression and function including analysis method of subcellular localization and dynamics of cellular macromolecules, along with the underlying basic concept. Corresponding criteria in the Diploma policy: See the curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/ |
| Course schedule |
|
Omnibus/15 periods total SASAKI Nobumitsu (4 periods) 1. Basics of microscopic analysis of subcellular localization and dynamics of cellular macromolecules 2. Research examples of subcellular localization and dynamics of RNA molecules 3. Research examples of subcellular localization and dynamics of proteins 4. Recent research topics about subcellular localization and dynamics of cellular macromolecules MATSUSHITA Yasuhiko (4 periods) 5. Analysis method of gene expression and function 6. Analysis method of gene expression (1) RNA 7. Analysis method of gene expression (2) Protein 8. Analysis method of gene function KITANO Yoshikazu (7 periods) 9. Outline of determining the structure of an organic compound 10. Infrared spectroscopy (IR) and Mass spectrometry (MS) 11. Nuclear magnetic resonance spectroscopy (NMR) 12. Strategy for determining the structure of an organic compound 13. Practice for determining the structure of an organic compound (1) 14. Practice for determining the structure of an organic compound (2) 15. Practice for determining the structure of an organic compound (3) |
| Prerequisites |
|
This lecture is an elective subject, and is one of the specialized subjects. Students need to preview and review for an amount of time as indicated in the student guidelines of TUAT, in addition to the attendance of lecture (30 hours). |
| Required Text(s) and Materials |
| Handouts (paper and/or electronic) will be provided if needed. |
| References |
|
Spectrometric Identification of Organic Compounds: Robert M. Silverstein, Francis X. Webster, David J. Kiemle, David L. Bryce (ISBN: 978-0-470-61637-6) Sankyo Publishing: Principle of Genetic Engineering (in Japanese) (ISBN: 978-4-7827-0637-4) Shujunsha: Fluorescence Imaging Revolution (ISBN: 978-4-7809-0835-0) |
| Assessment/Grading |
| Performance in lecture (Kitano; 10% total), examination (Kitano; 40% total), report assignments (Matsushita & Sasaki; 50% total) |
| Message from instructor(s) |
| We hope that this lecture will help you on your research in the future. |
| Course keywords |
| Infrared spectroscopy, Mass spectrometry, Nuclear magnetic resonance spectroscopy, Next generation sequencer, RNA-seq, Yeast hybrid method, Fluorescent microscope, Confocal laser microscope, Bimolecular fluorescence complementation method |
| Office hours |
| Questions can be asked after class, or please request an appointment by email. |
| Remarks 1 |
| Remarks 2 |
| 2019; S 27%, A 73% |
| Related URL |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 4/17/2020 2:08:10 PM |