| Course title | |||||
| 応用生命化学特論Ⅳ [Special Lecture on life and Biological Chemistory Ⅳ] | |||||
| Course category | Requirement | Credit | 1 | ||
| Department | Year | 1～ | Semester | 1st | |
| Course type | 1st | Course code | 05lc0110 | ||
| Instructor(s) | |||||
| 佐々木 信光, 大藤 道衛 [SASAKI Nobumitsu, OHTOH Michiei] | |||||
| Facility affiliation | Graduate School of Agriculture | Office | afjgxte/L1151 | Email address | |
| Course description |
| This course describes various methods for genetic analyses and their applications for cancer research, an introduction to next generation sequencing technologies and an overview of gene literacy education for promoting public understanding of life science and biotechnology. |
| Expected Learning |
|
Students who successfully complete this course will be able to: (1) Learn genetic and environmental factors in carcinogenesis. (2) Understand analytical methods for point mutations, SNPs and DNA methylation. (3) Discuss the prospects for new technologies of genetic analysis. Corresponding criteria in the Diploma policy: See the curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/ |
| Course schedule |
|
1. Genetic analysis of disease-related genes Introduction to methods for genetic analyses and their applications for cancer research. This course describes molecular analyses of cancer-related genes in hereditary and sporadic gastroenterological cancers. 1.1. Life science and genome 1.2. Disease-related genes 1.3. Genetic aspect of carcinogenesis 1.4. Methods for detecting point mutations and SNPs 1.5. Epigenetic aspects of carcinogenesis 1.6. Methods for DNA methylation analysis 2. Molecular analyses of nonculturable bacterial communities 2.1. Nonculturable bacteria 2.2. DNA analysis of bacterial communities 2.3. Data analysis 2.4. Metagenomic analysis 3. An Introduction to next generation sequencing technologies 3.1. Next generation sequencer and genome science 3.2. Single molecule sequencing technologies 3.3. The prospects for next generation sequencing technologies 4. Molecular medicine and literacy education 4.1 Biotechnology industry 4.2 Gene literacy education for public understanding of life science and biotechnology. |
| Prerequisites |
|
Knowledge of undergraduate level of molecular biology and gene technology is required. In addition to the lectures in the class, you are recommended to prepare and review the class using the standard amount of time as specified by the university for each class. |
| Required Text(s) and Materials |
| Handouts are available. |
| References |
| Green MR and Sambrook J : “Molecular cloning A loboratory manual” 4th ed. by Cold Spring Harbor Laboratory press (2012), Maria S. Poptsova, ed.: " Genome Analysis: Current Procedures and Applications" Caister Academic Press (2014) |
| Assessment/Grading |
| Learning attitude in the lecture (40%) and report (60%) |
| Message from instructor(s) |
| Any questions about the lecture are welcome. |
| Course keywords |
| Genetics、genomics、epigenetics、point mutation、SNP、digital PCR、microfluidic chip、paper-based microfluidic device、next generation sequencer、gene literacy education |
| Office hours |
| Thirty minutes after each lecture. |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 3/6/2020 2:41:02 PM |