Course title
遺伝子工学   [Gene Engineering]
Course category		Requirement		Credit	2
Department		Year	3～	Semester	1st
Course type	1st	Course code	01bn3021
Instructor(s)
松下 保彦   [MATSUSHITA Yasuhiko]
Facility affiliation	Center for Infectious Disease Epidemiology and Prevention Research	Office	Gene Reserch Center, East building, room E17	Email address

Course description

In the field of applied biological sciences, it is important to acquire analytical and insight skills to understand a series of life phenomena and biological functions, from molecules, cells and individuals to the activities and interactions of biological communities. For that purpose, it is important to know how in vivo molecules such as nucleic acids and proteins can be analyzed from the viewpoint of gene function. Genetic analysis is technically and knowledgeably the basis for food, plant protection, microbiology, and more. This course describes basic techniques relating to a recombinant DNA experiment, analysis of gene expression, and functional analysis of gene products.

Expected Learning

Learners who successfully complete this course will be able to: (1) Recognize the basic techniques for recombinant DNA experiments. (2) Make experimental plans for the research using recombinant DNA technologies including cloning and functional analysis of genes. (3) Understand the principle of representative experimental method used for analysis of gene expression and functional analysis of gene products. Corresponding criteria in the Diploma policy: See the curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/

Course schedule

1. DNA processing technology ・ Confirmation of basic knowledge (nucleic acid structure, etc.), principle of PCR method, primer design ・ DNA synthesis by reverse transcription reaction 2. DNA processing using PCR method (1) ・ Deletion, fusion, insertion, mutation ・ Primer design 3. DNA processing using PCR method (2) ・ Deletion, fusion, insertion, mutation (continued) ・ Processing of plasmid using PCR method ・ Primer design 4. Gene cloning method (1) ・ What is genetic engineering? ・ Genome, gene, clone, cloning, vector, plasmid 5. Gene cloning method (2) ・ Transformation of E. coli ・ Recombination of different DNA molecules 6. Gene cloning method (3) ・ Recombination of different DNA molecules (continued) ・ Detection of recombinant DNA molecules 7. Analysis method of gene expression (1) ・ Northern blot analysis ・ Western blot analysis, ELISA method 8. Analysis method of gene expression (2) ・ Real-time quantitative PCR 9. Analysis method of gene expression (3) ・ Real-time quantitative PCR method (continued) ・ Digital PCR method ・ Differential display method 10. Analysis method of gene expression (4) ・ Overview of nucleic acid analysis method ・ Microarray, DNA chip (GeneChip) 11. Analysis method of gene expression (5) ・ EST analysis ・ Principle of next-generation DNA sequencer ・ Gene expression analysis by next-generation DNA sequencer 12. Analysis method of gene expression (6) ・ Chromatin immunoprecipitation analysis by next-generation DNA sequencer ・ Overview of protein analysis method ・ Gene expression analysis by mass spectrometer 13. Functional analysis method for gene products (1) ・ Overview of gene function analysis method ・ Points for gene function analysis ・ Analysis method to change the amount of gene product ・ Other analysis methods 14. Functional analysis method for gene products (2) ・ Other analysis methods (continued) ・ Analysis method of interaction between protein and nucleic acid (Yeast one hybrid method, Southwestern method, chromatin immunoprecipitation analysis, Footprint method, gel shift assay) 15. Functional analysis method for gene products (3) ・ Analysis method of protein-protein interaction (Yeast two-hybrid method, far western method, FRET method, BiFC method, Immunoprecipitation method, use of mass spectrometer)

Prerequisites

It is preferable to have taken cell/genetics and molecular biology. In addition to 30 hours in the class, students are recommended to prepare for and revise the classes spending the standard amount of time as specified by the University.

Required Text(s) and Materials

The lecture will be given based on the lecture materials posted in the classroom etc.

References

Molecular Biology of the Gene (ISBN: 978-0-321-76243-6) Principles of genetic engineering (ISBN: 978-4-7827-0637-4) (Sankyo Publishing)

Assessment/Grading

Evaluate by examination (40%) and report or small test (60%). If the test cannot be conducted due to social conditions, a report (100%) will be used.

Message from instructor(s)

If you are interested, please study actively by reading reference books more and more. You are recommended to read the original text of reference books (in English), to get used to English if possible.

Course keywords

Cloning, gene amplification, gene introduction, vector, library, next generation genome analyzer, analysis of gene expression, function analysis of gene products

Office hours

Taking an appointment by e-mail or after class. Matsushita: ymatsu@cc.tuat.ac.jp

Remarks 1

【Please check the classroom of this course for any changes to the class schedule due to the spread of the new coronavirus infection. 】

Remarks 2

Distribution of past results are as follows. R2 (2020) S 33%, A 35%, B 20%, C 3%, D 7%, E 2% R1 (2019) S 30%, A 51%, B 12%, C 4%, D 0%, E 3% H30 (2018) S 34%, A 38%, B 16%, C 8%, D 0%, E 4% H29 (2017) S 38%, A 28%, B 18%, C 6%, D 4%, E 6% H28 (2016) S 24%, A 28%, B 18%, C 20%, D 8%, E 1% H27 (2015) S 29%, A 12%, B 41%, C 17%, D 0%, E 1% H26 (2014) S 23%, A 31%, B 25%, C 16%, D 3%, E 2% H25 (2013) S 19%, A 19%, B 25%, C 19%, D 8%, E 10% H24 (2012) S 16%, A 17%, B 14%, C 24%, D 15%, E 14% H23 (2011) S 9%, A 20%, B 19%, C 39%, D 13% H22 (2010) S 12%, A 17%, B 27%, C 23%, D 21% H21 (2009) S 6%, A 42%, B 33%, C 4%, D 15% H20 (2008) S 3%, A 32%, B 50%, C 11%, D 4% H19 (2007) S 15%, A 24%, B 30%, C 20%, D 11% H18 (2006) S 27%, A 33%, B 21%, C 13%, D 6% H17 (2005) S 9%, A 26%, B 32%, C 21%, D 12%

Related URL

Lecture Language

Japanese

Language Subject

Last update

2/2/2021 12:49:43 AM