| Course title | |||||
| 応用生物科学専門実験Ⅱ [Laboratory Work of Applied Biological Science Ⅱ] | |||||
| Course category | Requirement | Credit | 1 | ||
| Department | Year | 3～ | Semester | 1st | |
| Course type | 1st | Course code | 01bn3040 | ||
| Instructor(s) | |||||
| 小松 健, 松下 保彦 [KOMATSU Ken, MATSUSHITA Yasuhiko] | |||||
| Facility affiliation | Faculty of Agriculture | Office | afjgxte/L1151 | Email address | |
| Course description |
| Students will learn basic technique of DNA cloning and concept of that throughout the course, and ultimately master basic genetic engineering technique. |
| Expected Learning |
|
Students should master basic technique of DNA cloning and learn the concept of gene modification throughout the course, and ultimately master basic genetic engineering technique, think over what’s behind in the experimental results and underlying mechanism. Corresponding criteria in the Diploma policy: See the curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/ |
| Course schedule |
|
A class：Students will perform following experiment. Digest λ phage-derived DNA with a restriction enzyme, ligate those fragments into a plasmid vector and transform E. coli bacterial cells. Isolate single colonies by streaking a plate. Purify DNA of interest from E. coli competent cells and confirm that exogenous DNA (DNA of interest) was inserted by (i) digestion with restriction enzyme followed by SDS-PAGE, (ii) determination base sequence. B class：Purify chromosomal DNA from thale-cress and confirm recombination of genes by using PCR analysis. Students will also purify genomic DNA from Bacillus subtilis and determine the purity of DNA. |
| Prerequisites |
| In addition to the lectures in the class, you are recommended to prepare and review the class using the standard amount of time as specified by the university for each class. |
| Required Text(s) and Materials |
| Text will be provided in class. Students should also prepare ‘Precaution against accidents and first-aid treatment (Kagaku-Dojin)’ in advance. |
| References |
| Molecular Cloning（Third Edition）,Cold Spring Harbor Laboratory Press,NewYork |
| Assessment/Grading |
| Grade will be decided by the report submitted during the course (100%). |
| Message from instructor(s) |
| Course keywords |
| Office hours |
| Please ask faculties in charge of the experiments. |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 1/25/2021 7:31:58 PM |