| Course title | |||||
| 応用生物科学専門実験Ⅲ [Laboratory Work of Applied Biological Science Ⅲ] | |||||
| Course category | Requirement | Credit | 1 | ||
| Department | Year | 3～ | Semester | 1st | |
| Course type | 1st | Course code | 01bn3041 | ||
| Instructor(s) | |||||
| 小松 健, 松下 保彦 [KOMATSU Ken, MATSUSHITA Yasuhiko] | |||||
| Facility affiliation | Faculty of Agriculture | Office | afjgxte/L1151 | Email address | |
| Course description |
|
A class：Students will perform experiment relevant to the field of natural products organic chemistry. B class：Analysis of gene function using drosophila and purification, identification and analysis of protein of interest by using specific antibody. |
| Expected Learning |
|
At the end of the course, students will develop their background and gain basic knowledge in natural products organic chemistry and embryology. Corresponding criteria in the Diploma policy: See the curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/ |
| Course schedule |
|
A class: (1) Isolation and purification of organic compound. Isolate and purify material of interest from biological sample by liquid-liquid partition or chromatography, and analyze the property of the constituent by thin-layer chromatography and gas chromatography. (2) Determination of the structure of organic compound by NMR, MS and IR. (3) Organic synthesis reaction. Students will prepare bioactive compound and its derivatives by using basic organic synthesis reaction. B class: (1) Analysis of genetic function of mutant drosophila. (2) Analysis of the gene expression system with reporter gene method. (3) Analysis of ectopic expression of gene in mutant fly. (4) Searching gene of interest using database. (5) Analysis and collection of protein of interest by immunoprecipitation and other precipitation system with selected binding affinity. (6) Confirmation of protein of interest by western blotting and specific antibody. |
| Prerequisites |
| Students in B class should understand the objective of this course as well as ultimate goal of this experimental course, developed by our overall experimental results. |
| Required Text(s) and Materials |
| Students in A class should prepare ‘Precaution against accidents and first-aid treatment (Kagaku-Dojin)’ and students in B class should prepare 'Illustrated Biological Experiment' (Shu-jun sya) in advance. |
| References |
| It will be announced in the class. |
| Assessment/Grading |
| Grade will be decided by the combination of the contribution to the experiment and the report submitted during the course. |
| Message from instructor(s) |
| Course keywords |
| Office hours |
| It will be announced in the class. |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 1/25/2021 7:32:08 PM |