Course title
生命工学実験Ⅰ   [Experiment for Biotechnology and Life Science Ⅰ]
Course category technology speciality courses  Requirement   Credit 4 
Department   Year 24  Semester 1st 
Course type 1st  Course code 022176
Instructor(s)
平田 美智子   [HIRATA Michiko]
Facility affiliation Faculty of Engineering Office afjgxte/L1151  Email address

Course description
・Goal and theme
This experiment aim is to master basic experimental techniques based on essential study for engineering science. We will study wide range of biotechnology techniques and form the right solution of research data. Topics include organic chemistry practice, physical chemistry practice, molecular biology practice, biochemistry practice, and instrumental analysis.

・Outline of class
In molecular biology - biochemistry practice, we will study protein purification method and enzyme characteristics. In organic chemistry - physical chemistry practice, we will study enzyme reaction kinetics and how to use a spectrophotometer. In addition, we will learn NMR and Infrared spectrophotometric measurement in instrumental analysis.
Expected Learning
Course schedule
* Course description
1. Introduction and risk management
2. Purification of phycocyanin 1
3. Purification of phycocyanin 2
4. Enzyme characteristics (Substrate specificity)
5. Enzyme characteristics (Temperature specificity 1)
6. Enzyme characteristics (Temperature specificity 2)
7. pK value measurement from visible absorption spectrum data
8. Ester hydrolysis reaction rate constant and energy of activation
9. Oxidation-reduction potential 1
10. Oxidation-reduction potential 2
11. Phase transition
12. Thermal transformation
13. Liquid NMR measurement
14. Infrared spectrophotometric measurement
15. Identification of unknown substance
Prerequisites
You are required to have related subject’s knowledge.
Required Text(s) and Materials
Experiment for Biotechnology and life Science I textbook
References
We will show you appropriate reference books as needed.
Assessment/Grading
Total evaluation with Experiment participation, Experiment notes, Reports
Message from instructor(s)
You will work collaboratively throughout the course to solve problems and work on your reports. You will find a delight in making experiments in this course.
Course keywords
Organic chemistry, physical chemistry, molecular biology, biochemistry, and instrumental analysis.
Office hours
Remarks 1
Remarks 2
Related URL
Lecture Language
Language Subject
Last update
4/1/2021 3:45:45 PM