| Course title | |||||
| 生命工学実験Ⅰ [Experiment for Biotechnology and Life Science Ⅰ] | |||||
| Course category | technology speciality courses | Requirement | Credit | 4 | |
| Department | Year | 2～4 | Semester | 1st | |
| Course type | 1st | Course code | 022176 | ||
| Instructor(s) | |||||
| 平田 美智子 [HIRATA Michiko] | |||||
| Facility affiliation | Faculty of Engineering | Office | afjgxte/L1151 | Email address | |
| Course description |
|
・Goal and theme This experiment aim is to master basic experimental techniques based on essential study for engineering science. We will study wide range of biotechnology techniques and form the right solution of research data. Topics include organic chemistry practice, physical chemistry practice, molecular biology practice, biochemistry practice, and instrumental analysis. ・Outline of class In molecular biology - biochemistry practice, we will study protein purification method and enzyme characteristics. In organic chemistry - physical chemistry practice, we will study enzyme reaction kinetics and how to use a spectrophotometer. In addition, we will learn NMR and Infrared spectrophotometric measurement in instrumental analysis. |
| Expected Learning |
| Course schedule |
|
* Course description 1. Introduction and risk management 2. Purification of phycocyanin 1 3. Purification of phycocyanin 2 4. Enzyme characteristics (Substrate specificity) 5. Enzyme characteristics (Temperature specificity 1) 6. Enzyme characteristics (Temperature specificity 2) 7. pK value measurement from visible absorption spectrum data 8. Ester hydrolysis reaction rate constant and energy of activation 9. Oxidation-reduction potential 1 10. Oxidation-reduction potential 2 11. Phase transition 12. Thermal transformation 13. Liquid NMR measurement 14. Infrared spectrophotometric measurement 15. Identification of unknown substance |
| Prerequisites |
| You are required to have related subject’s knowledge. |
| Required Text(s) and Materials |
| Experiment for Biotechnology and life Science I textbook |
| References |
| We will show you appropriate reference books as needed. |
| Assessment/Grading |
| Total evaluation with Experiment participation, Experiment notes, Reports |
| Message from instructor(s) |
| You will work collaboratively throughout the course to solve problems and work on your reports. You will find a delight in making experiments in this course. |
| Course keywords |
| Organic chemistry, physical chemistry, molecular biology, biochemistry, and instrumental analysis. |
| Office hours |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Language Subject |
| Last update |
| 4/1/2021 3:45:45 PM |