| Course title | |||||
| 生命工学実験Ⅲ [Experiment for Biotechnology and Life Science Ⅲ] | |||||
| Course category | technology speciality courses | Requirement | Credit | 4 | |
| Department | Year | 3～4 | Semester | 1st | |
| Course type | 1st | Course code | 023171 | ||
| Instructor(s) | |||||
| 平田 美智子 [HIRATA Michiko] | |||||
| Facility affiliation | Faculty of Engineering | Office | afjgxte/L1151 | Email address | |
| Course description |
|
・Goal and Theme This experiment aim is to master basic experimental techniques based on essential study for engineering science. We will study wide range of biotechnology techniques and form the right solution of research data. Topics include organic chemistry practice, physical chemistry practice, molecular biology practice, biochemistry practice, and instrumental analysis in biotechnology. ・Outline of class In molecular biology - biochemistry practice, we will study mammals cell culture and gene transfer method. In organic chemistry - physical chemistry practice, we will study protein characteristics in thermal stability, 3D structure and recombinant protein method. In addition, we will learn basic biological instrumental analysis. |
| Expected Learning |
| Course schedule |
|
1. Introduction and risk management 2. Mammals cell culture 3. Gene transfer1 4. Gene transfer2 5. Gene transfer3 6. Gene transfer4 7. Thermal stability of protein 8. Protein structural analysis algorithm1 9. Protein structural analysis algorithm2 10. Expression of recombinant protein 11. Purification of recombinant protein 12. DNA sequence analysis 1 13. DNA sequence analysis 2 14. HPLC (High Performance Liquid Chromatography) 14. Southern hybridization 1 15. Southern hybridization 2 |
| Prerequisites |
| You are required to have related subject’s knowledge. |
| Required Text(s) and Materials |
| Experiment for Biotechnology and life Science III textbook |
| References |
| We will show you appropriate reference books as needed. |
| Assessment/Grading |
| Total evaluation with Experiment participation, Experiment notes, Reports |
| Message from instructor(s) |
| You will work collaboratively throughout the course to solve problems and work on your reports. You will find a delight in making experiments in this course. |
| Course keywords |
| Organic chemistry, physical chemistry, molecular biology, biochemistry, and instrumental analysis in biotechnology. |
| Office hours |
| Remarks 1 |
| Remarks 2 |
| Related URL |
| Lecture Language |
| Language Subject |
| Last update |
| 4/1/2021 3:52:25 PM |