Course title
生命工学実験Ⅲ   [Experiment for Biotechnology and Life Science Ⅲ]
Course category technology speciality courses  Requirement   Credit 4 
Department   Year 34  Semester 1st 
Course type 1st  Course code 023171
Instructor(s)
平田 美智子   [HIRATA Michiko]
Facility affiliation Faculty of Engineering Office afjgxte/L1151  Email address

Course description
・Goal and Theme
This experiment aim is to master basic experimental techniques based on essential study for engineering science. We will study wide range of biotechnology techniques and form the right solution of research data. Topics include organic chemistry practice, physical chemistry practice, molecular biology practice, biochemistry practice, and instrumental analysis in biotechnology.

・Outline of class
In molecular biology - biochemistry practice, we will study mammals cell culture and gene transfer method. In organic chemistry - physical chemistry practice, we will study protein characteristics in thermal stability, 3D structure and recombinant protein method. In addition, we will learn basic biological instrumental analysis.
Expected Learning
Course schedule
1. Introduction and risk management
2. Mammals cell culture
3. Gene transfer1
4. Gene transfer2
5. Gene transfer3
6. Gene transfer4
7. Thermal stability of protein
8. Protein structural analysis algorithm1
9. Protein structural analysis algorithm2
10. Expression of recombinant protein
11. Purification of recombinant protein
12. DNA sequence analysis 1
13. DNA sequence analysis 2
14. HPLC (High Performance Liquid Chromatography)
14. Southern hybridization 1
15. Southern hybridization 2
Prerequisites
You are required to have related subject’s knowledge.
Required Text(s) and Materials
Experiment for Biotechnology and life Science III textbook
References
We will show you appropriate reference books as needed.
Assessment/Grading
Total evaluation with Experiment participation, Experiment notes, Reports
Message from instructor(s)
You will work collaboratively throughout the course to solve problems and work on your reports. You will find a delight in making experiments in this course.
Course keywords
Organic chemistry, physical chemistry, molecular biology, biochemistry, and instrumental analysis in biotechnology.
Office hours
Remarks 1
Remarks 2
Related URL
Lecture Language
Language Subject
Last update
4/1/2021 3:52:25 PM