| Course title | |||||
| 生体分子化学Ⅰ [Biomolecular and Biomaterial Chemistry Ⅰ] | |||||
| Course category | Requirement | Credit | 2 | ||
| Department | Year | 1～ | Semester | 1st | |
| Course type | 1st | Course code | 05lc0101 | ||
| Instructor(s) | |||||
| 北野 克和, 岡田 洋平, 佐々木 信光, 松下 保彦 [KITANO Yoshikazu, OKADA Yohei, SASAKI Nobumitsu, MATSUSHITA Yasuhiko] | |||||
| Facility affiliation | Faculty of Agriculture | Office | Email address | ||
| Course description |
|
This course provides students with the skills of determining the structure of low molecular-weight organic compounds. This course also provides students with the basics on analysis methods of gene expression and function including those of subcellular localization and dynamics of cellular macromolecules. We also describe various analytical methods on gene expression and function along with the basic idea underlying them. In addition, we explain the techniques, examples, and recent information about researches of subcellular localization and dynamics of cellular macromolecules. |
| Expected Learning |
|
Learners who succesfully complete this course will be able to identify the structure of low molecular-weight organic compounds by analayzing IR, MS, and NMR spectra. Learners who succesfully complete this course will be able to understand the principle of various analytical methods concerning gene expression and function including analysis method of subcellular localization and dynamics of cellular macromolecules, along with the underlying basic concept. Corresponding criteria in the Diploma policy: See the curriculum maps. https://www.tuat.ac.jp/campuslife_career/campuslife/policy/ |
| Course schedule |
|
Omnibus/15 periods total KITANO Yoshikazu (3 periods) 1. Outline of determining the structure of an organic compound 2. Infrared spectroscopy (IR) and Mass spectrometry (MS) 3. Nuclear magnetic resonance spectroscopy (NMR) OKADA Yohei (4 periods) 4. Strategy for determining the structure of an organic compound 5. Practice for determining the structure of an organic compound (1) 6. Practice for determining the structure of an organic compound (2) 7. Practice for determining the structure of an organic compound (3) SASAKI Nobumitsu (4 periods) 8. Basics of microscopic analysis of subcellular localization and dynamics of cellular macromolecules 9. Research examples of subcellular localization and dynamics of RNA molecules 10. Research examples of subcellular localization and dynamics of proteins 11. Recent research topics about subcellular localization and dynamics of cellular macromolecules MATSUSHITA Yasuhiko (4 periods) 12. Analysis method of gene expression and function Outline various research methods for analyzing gene expression and function. Explain the method of overexpressing genes (overview and enhancer trap method). 13. Analysis method of gene expression (1) Explain the principle of EST analysis and SAGE method. 14. Analysis method of gene expression (2) Explain the RNA analysis method using the next-generation sequencer. 15. Analysis method of gene expression and function Explain the protein analysis method using a mass spectrometer. Explain the chromatin immunoprecipitation analysis. Explain the method of overexpressing genes (activation tagging method). |
| Prerequisites |
|
This lecture is an elective subject, and is one of the specialized subjects. Students need to preview and review for an amount of time as indicated in the student guidelines of TUAT, in addition to the attendance of lecture (30 hours). |
| Required Text(s) and Materials |
| Handouts (paper and/or electronic) will be provided if needed. |
| References |
|
Spectrometric Identification of Organic Compounds: Robert M. Silverstein, Francis X. Webster, David J. Kiemle, David L. Bryce (ISBN: 978-0-470-61637-6) Sankyo Publishing: Principle of Genetic Engineering (in Japanese) (ISBN: 978-4-7827-0637-4) Shujunsha: Fluorescence Imaging Revolution (ISBN: 978-4-7809-0835-0) |
| Assessment/Grading |
| Performance in lecture (Kitano & Okada; 10% total), examination (Kitano & Okada; 40% total), report assignments (Matsushita & Sasaki; 50% total) |
| Message from instructor(s) |
| We hope that this lecture will help you on your research in the future. |
| Course keywords |
| Infrared spectroscopy, Mass spectrometry, Nuclear magnetic resonance spectroscopy, Next generation sequencer, RNA-seq, Fluorescent microscope, Confocal laser microscope, Bimolecular fluorescence complementation method |
| Office hours |
| Questions can be asked after class, or please request an appointment by email. |
| Remarks 1 |
| Remarks 2 |
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2021; S 21%, A 79% 2020; S 13%, A 71%, B 16% 2019; S 27%, A 73% |
| Related URL |
| Lecture Language |
| Japanese |
| Language Subject |
| Last update |
| 2/1/2022 7:55:11 AM |